Knock-down effect of HSP70 antisense oligos. Hep-2 cells were transfected with HSP70 antisense oligos (AS) or control oligos (sense oligos and random oligos) as described under materials and methods. After incubation for 48 h, cells were harvested, lysed. Western-blotted (WB) with the corresponding antibodies was carried out to show the knock-down effect of AS. AS-1 has the best knock-down effect of all 3 oligos.