Analysis of the expression profile of Dickkopf-1 gene in human glioma and the association with tumor malignancy

Table 1 Levels of DKK-1 expression were detected in the culture medium and cell lysate of all 14 cancer cell lines by ELISA

Cancer cell lines and control	Concentration of DKK1 (pg/ml)
*Normal cell* s
Human astrocytes	0	0
*Low-grade glioma cell line*
SHG-44	0	0
*Medulloblastoma cell line*
D341	0	0
*Glioblastoma cell lines*	Culture medium*	Cell lysate**
U251	18238	4917
SF767	5760	729
T98G	1558	258
UW-28	2390	45
MGR1	1089	151
MGR2	3826	434
MGR3	3901	375
SKI-N2	766	260
SKMG-1	6691	2192
SKMG-4	301	72
UWR7	5290	910
SF295	8628	1780

* and ** indicate the respective concentration of DKK-1 protein tested in the culture medium and cell lysate.

ISSN: 1756-9966