Figure 6

Effects of TGF-β1 on Smad 2 and 3 phosphorylation in the mesothelial cells. The HPMCs were grown in serum-free medium with or without 5 ng/mL TGF-β1 treatment for up to 24 h. Total cellular protein was then extracted and subjected to Western blot analysis. A, Expression of phosphorylated Smad 2 protein. B, Expression of phosphorylated Smad 3 protein. C, Total Smad 2/3 protein.