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Table 1 Candidate reference genes included in the TaqMan Endogenous Control Assay.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Gene name Gene symbol Assay ID Size (bp) Function
18S Ribosomal RNA 18S Hs99999901_s1 187 Part of a ribosomal subunit
Phosphoglycerate kinase-1 PGK1 Hs99999906_m1 75 Key enzyme in glycolysis
Β-Actin ACTB Hs99999903_m1 171 Cytoskeletal structural protein
Polymerase (RNA) II polypeptide A POLR2A Hs00172187_m1 61 Catalyzes the RNA synthesis from DNA
Beta-2-microglobulin B2M Hs99999907_m1 75 Beta-chain of major histocompatibility complex class I molecules
Peptidyl-prolyl isomerase/cyclophilin A PPIA Hs99999904_m1 98 Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerating folding
Glyceraldehyd-3-phosphate dehydrogenase GAPDH Hs99999905_m1 122 Dehydrogenase, oxidoreductase in glycolysis and gluconeogenesis
Acidic ribosomal phosphoprotein P0 RPLP0 Hs99999902_m1 105 Ribosome biogenesis and assembly
Β-Glucuronidase GUSB Hs99999908_m1 81 Glycoprotein, degradation of dermatan and keratin sulfates
Transcription factor IID, TATA box binding protein TBP Hs99999910_m1 127 TATA binding protein, general RNA polymerase II transcription factor
Hydromethylbilane synthase HMBS Hs00609297_m1 64 Heme synthesis, porphyrin metabolism
Transferrin receptor (p90, CD71) TFRC Hs99999911_m1 105 Cellular uptake of iron
Hypoxanthine-phosphoribosyl-transferase 1 HPRT1 Hs99999909_m1 100 Glycosyltransferase, purine synthesis in salvage pathway
Ubiquitin C UBC Hs00824723_m1 71 Protein degradation
Importin 8 IPO8 Hs00183533_m1 71 Function in nuclear protein import
Tyrosine 3 monooxygenase activation protein, zeta polypeptide YWHAZ Hs00237047_m1 70 Signal transduction by binding to phosphorylated serine residues on a variety of signalling molecules