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Table 2 Cycle threshold (Ct) values of candidate reference genes divided in the four tissue groups.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Gene symbol Non-metastatic colon cancer Metastatic colon cancer
  Tumour Normal Tumour Normal
  Mean SD N Mean SD N Mean SD N Mean SD N
18S 8,095 0,546 18 8,440 1,066 18 8,800 1.066 20 9,408 2,035 20
ACTB 20,003 0,765 18 19,949 1,209 18 20,363 1.209 20 20,578 2,673 20
B2M 17,050 0,996 18 17,041 1,002 18 17,217 1.002 20 17,085 1,632 20
GAPDH 18,503 0,722 18 19,502 1,044 18 19,211 1.044 20 20,145 2,541 20
GUSB 23,274 0,375 18 24,081 0,865 18 23,564 0.865 20 24,060 1,981 20
HMBS 25,328 0,736 18 26,577 0,974 18 25,963 0.974 20 27,030 2,436 20
HPRT1 22,795 0,814 18 24,183 0,750 18 23,320 0.750 20 24,264 1,849 20
IPO8 24,575 0,469 18 25,084 0,780 18 25,099 0.780 20 25,529 2,108 20
PGK1 20,322 1,054 18 21,151 1,012 18 20,996 1.011 20 21,573 3,257 20
POLR2A 24,007 0,634 18 24,508 1,061 18 24,933 1.061 20 25,330 2,590 20
PPIA 17,081 0,485 18 18,241 0,906 18 17,506 0.906 20 18,335 1,724 20
RPLP0 19,706 0,637 18 20,647 0,952 18 20,319 0.952 20 21,081 2,002 20
TBP 26,157 0,577 18 26,860 1,035 18 26,649 1.035 20 27,110 2,797 20
TFRC 21,774 0,926 18 23,334 1,030 18 22,679 1.030 20 23,663 2,303 20
UBC 21,285 0,675 18 21,771 1,046 18 21,532 1.046 20 22,044 2,180 20
YWHAZ 23,933 0,723 18 25,041 1,275 18 24,457 1.275 20 25,401 2,174 20