Figure 5

Ad-bFGF-siRNA improves GJIC between U251 cells. GJIC was assessed in U251 cells infected with Ad-bFGF-siRNA (100 MOI) for 48 h compared to untreated U251 cells using scrape loading dye transfer assays. A) In untreated cells, Lucifer Yellow was restricted to the cells at the border of the scraped line with only minimal transfer of Lucifer Yellow to neighboring cells. B) In Ad-bFGF-siRNA U251 cells, an increase in the transfer of Lucifer Yellow between cells was detected.