Figure 4

The capacity of CCL3 and CCL20-recruited F4/80^- B220^-CD11c⁺ cells to enhance allogeneic MLR. Allogeneic MLR were performed using splenic T cells purified from B6 mice as responder cells. Fresh and cultured F4/80^-B220^-CD11c⁺ cells were treated with MMC to arrest cell proliferation and were used as stimulator cells at the indicated cell numbers, respectively. Macrophage were used as controls. T cell proliferation was determined with MTT after 5 days of culture. Results are expressed as the mean ± SD of triplicate cultures. All data are representative of three independent experiments.