Figure 4

Angiogenesis pattern of eutopic endometrium (A, D, G), and endometriotic lesions after 15 days (B, E, H) and 30 days (C, F, I). The immunoreactivity of VEGF and Flk-1 were detected mainly in the cytoplasm of endothelial (arrows) and glandular epithelial cells (arrowheads) but also in stromal cells (asterisks) in both eutopic and ectopic endometrial tissues. As expected, VEGF and Flk-1 immunoreactions were more abundant in endometriosis than in the eutopic endometrium. The distribution of the ED-1-positive macrophages was observed in the cells in the stroma, concentrated around the glands. There were more activated macrophages in samples of endometriosis than in eutopic endometrium (black squares). Magnification × 400.