Figure 5
From: Generation of functional CD8+ T Cells by human dendritic cells expressing glypican-3 epitopes
Processing of HLA-A2-restricted GPC-3 epitopes by mRNA transfected DC. T cell pools were expanded firstly by a round of stimulation with autologous, irradiated, matured DC pulsed with 1 μM GPC-3 or irrelevant control peptides, or DC transfected with either GPC-3 mRNA or eGFP mRNA as control, followed by a second round of stimulation with autologous, irradiated, matured DC pulsed with 1 μM GPC-3 or irrelevant control peptides. T cell proliferation was assessed by thymidine incorporation, at a stimulator to responder ratio of 1:10. * p < 0.05 and ** p < 0.01 compared to T cells stimulated in the first round by eGFP mRNA transfected DC; error bars reflect standard error of mean of 3 experiments.