Tumor cell killing effect of Ad.hTERT-E1A-TK on NSCLC NCIH460 cells. A. Crystal violet staining of tumor cells after infection with different adenoviral vectors. NSCLC NCIH460 cells were plated into 24-well plates and treated with different doses of adenoviral vectors or prodrug or untreated as indicated in figure. 5 days later the plates were stained with crystal violet. B. CCK-8 assay for surviving cells after infection with Ad.hTERT-E1A-TK. NSCLC NCIH460 and A549 cells, and cervical carcinoma Hela cellswere plated into 96-well plates and infected by 10 MOI of Ad.hTERT-E1A-TK with or without 0.5 μg/ml GCV. 5 days later the surviving cells were quantified with CCK-8 assay and normalized by untreated cells.