Figure 4

Inhibition of tumor angiogenesis, apoptosis and proliferation by VEGF silencing plus DDP in vivo. A) Representative photographs of the tumor sections examined by immunohistochemical staining for CD31 showing tumor vasculature (×400 magnification). Each bar represents the average vessel number for each group, expressed as mean ± SD. *P < 0.05 versus pshVEGF or DDP. B) Representative photographs of the tumor sections examined by TUNEL assay. TUNEL-positive cell nuclei (green) were observed under a fluorescence microscope (×400). Each bar represents the 'apoptosis index', expressed as mean ± SD.*P < 0.05 versus pshVEGF or DDP. C) Representative photographs of the tumor sections examined by immunohistochemical staining for PCNA (×400). The assessment of PCNA was based on a nuclear staining pattern. Each bar represents the ratio of PCNA positive cells to the total number of cells for each group, expressed as mean ± SD. *P < 0.05 versus pshVEGF or DDP.