Survivin Expression in wild type vs. p53 null cancer cell sublines. A. HCT116 and HCT116p53-/-. B. MCF-7 with wild type p53 and MDA-MB-231 with mutant p53. C. Kms11 with wild type p53 and RPMI-8226 with mutant p53. Sub-confluent cells were lysed, and the cell lysates were used to determine survivin expression by western blots. Actin is the internal control for total protein loading. The expression of survivin in wild type p53 cells was set at 1 and relative survivin expression is shown after normalization with the actin internal control.