siRNA knockdown of Slug and overexpression of Slug with the invasive potential in EHC cells. Cells were seeded in the upper chamber in medium supplemented with 5% FCS. Results are reported as percent migration ± SD compared with untreated cells. Experiments were carried out twice in triplicate. A Slug silencing inhibits invasion potention of QBC939 cells in Matrigel-coated invasion chambers. B Slug overexpression promotes invasive potential in FRH 0201 cells in Matrigel-coated invasion chambers.