Figure 4

Wortmannin inhibits adaphostin (ADA)-induced translocation of Nrf2 into the nucleus. NCI-H522 cells were pretreated 30 minutes with 500 nM wortmannin where indicated, followed by 4 hour incubation with 1 μM ADA and stained with Nrf2 and FITC-conjugated antibodies. Nuclei were counter-stained with the fluorescence dye DAPI. Data are representative of two independent experiments.