Figure 1

mRNA and protein expression levels of TLRs (TLR1 through TLR10) in human breast cancer cell line MDA-MB-231. A, semi-quantitative RT-PCR of TLRs in MDA-MB-231. The GAPDH mRNA was amplified as control. B, real-time RT-PCR of TLRs in MDA-MB-231. The expression of TLR3 was normalized to 1.0 as it was expressed weakest among all TLRs. C, Flow cytometry of TLRs protein expression levels in MDA-MB-231. All results are representative of three separate experiments.