Table 1 PCR primers of human TLRs
Genes | Primer sequence (5'-3') | Amplification size (bp) |
|---|---|---|
TLR1 | For: TCTGGTACACGCATGGTC | 517 bp |
Rev: ATGGGTGGGAAACTGAAT | ||
TLR2 | For: AACTTACTGGGAAATCCTTAC | 264 bp |
Rev: AAAAATCTCCAGCAGTAAAAT | ||
TLR3 | For: GCATTTGTTTTCTCACTCTTT | 131 bp |
Rev: TTAGCCACTGAAAAGAAAAAT | ||
TLR4 | For: CGAGGAAGAGAAGACACCAGT | 106 bp |
Rev: CATCATCCTCACTGCTTCTGT | ||
TLR5 | For: AGCTTCAACTATATCAGGACA | 383 bp |
Rev: TGGTTGGAGGAAAAATCTAT | ||
TLR6 | For: CTTCCATTTTGTTTGCCTTAT | 123 bp |
Rev: AGCGGTAGGTCTTTTGGAAC | ||
TLR7 | For: AAACTCCTTGGGGCTAGATG | 149 bp |
Rev: AGGGTGAGGTTCGTGGTGTT | ||
TLR8 | For: CTGTGAGTTATGCGCCGAAGA | 246 bp |
Rev: TGGTGCTGTACATTGGGGTTG | ||
TLR9 | For: CGCCCTGCACCCGCTGTCTCT | 168 bp |
Rev: CGGGGTGCTGCCATGGAGAAG | ||
TLR10 | For: AGAAGAAAGGGAACTGATGAC | 279 bp |
Rev: CCTGCCAGTAAATACCAAGT | ||
GAPDH | For: GGATTTGGTCGTATTGGG | 205 bp |
Rev: GGAAGATGGTGATGGGATT |