Table 1 SA-β-gal assay and cell number after α-amylase treatment in F344 and Lewis cells

SA-β-gal assay

SA-β-gal-positive cells (%)

SA-β-gal-positive cells (%)

SA-β-gal-positive cells (%)

Control (H ₂ O)

11.94 ± 1.81

27.35 ± 3.28

33.82 ± 1.48

5 U/ml α-amylase

13.86 ± 1.41

37.15 ± 3.19

34.12 ± 3.20

50 U/ml α-amylase

11.83 ± 2.39

39.48 ± 3.47*

29.81 ± 2.78

n.s.

*H₂O vs. 50 U/ml

n.s.

F344, P1

F344, P2

Lewis, P2

Cell counts

Number of cells/well

Number of cells/well

Number of cells/well

Control (H ₂ O)

17,250 ± 1,377

4,500 ± 577

4,188 ± 567

5 U/ml α-amylase

17,958 ± 1,514

3,958 ± 240

5,292 ± 163

50 U/ml α-amylase

11,833 ± 870*

2,371 ± 344

4,483 ± 464

*H₂O vs. 50 U/ml

n.s.

n.s.