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Table 1 SA-β-gal assay and cell number after α-amylase treatment in F344 and Lewis cells

From: Salivary α-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells

  F344, P1 F344, P2 Lewis, P2
SA-β-gal assay SA-β-gal-positive cells (%) SA-β-gal-positive cells (%) SA-β-gal-positive cells (%)
Control (H 2 O) 11.94 ± 1.81 27.35 ± 3.28 33.82 ± 1.48
5 U/ml α-amylase 13.86 ± 1.41 37.15 ± 3.19 34.12 ± 3.20
50 U/ml α-amylase 11.83 ± 2.39 39.48 ± 3.47* 29.81 ± 2.78
  n.s. *H2O vs. 50 U/ml n.s.
  F344, P1 F344, P2 Lewis, P2
Cell counts Number of cells/well Number of cells/well Number of cells/well
Control (H 2 O) 17,250 ± 1,377 4,500 ± 577 4,188 ± 567
5 U/ml α-amylase 17,958 ± 1,514 3,958 ± 240 5,292 ± 163
50 U/ml α-amylase 11,833 ± 870* 2,371 ± 344 4,483 ± 464
  *H2O vs. 50 U/ml n.s. n.s.
  1. α-Amylase (50 U/ml) decreased the number of cells only in P1-F344-cells, but not in P2-F344- and P2-Lewis-cells. Proportion of SA-β-gal-positive cells did not correlate with cell number, as this amount of cells was not altered in P1-F344 cells, but significantly increased in P2-F344 cells after 50 U/ml α-amylase. No difference at all was observed in Lewis-cells (P2) and after 5 U/ml α-amylase. Mean and SEM are shown for three wells per group (cell counts) or 6-9 sections (SA-β-gal assay). Significant differences (p < 0.05) vs. control cells (One-way-ANOVA and Bonferroni for selected pairs) are indicated by asterisk.