Induction of CLU in a time and dose dependent fashion by TX. A. Western analysis showing s-CLU expression after 6 h treatment with TX. Induction of s-CLU is evident in chemo-sensitive KF cells when treated with high doses of TX but not in KF-TX, in which the high levels of s-CLU remained unchanged. Under these conditions, KF cells' viability decreased. B. Western analysis showing s-CLU expression in cell extracts (upper panel) and culture media (lower panel) after 48 h treatment with TX. CLU increased in TX-sensitive KF cells at different doses while CLU secretion was inhibited. At difference, expression and secretion of CLU was unchanged in the TX-resistant cells. Only at very high concentrations of TX a consistent down-regulation of s-CLU in the media was detectable. Ponceau S staining of the blot is provided to show equal loading of the protein samples because Actin and tubulin are responding to TX. The data shown are representative of four independent experiments.