Sample | GAPDH CT valve average | Zfx CT value average | 2-△△CT average |
---|
scr-siRNA | 16.34 ± 0.06 | 25.89 ± 0.04 | 1.00 ± 0.06 |
Zfx-siRNA | 16.1 ± 0.02 | 28.27 ± 0.10 | 0.16 ± 0.001 |
- Table 1:CT values of GAPDH and Zfx detected by real-time quantitative PCR. The Zfx mRNA expression levels in U251 cells at the 5th day after infection with Zfx-siRNA lentivirus and NC lentivirus were analyzed by 2-△△CT method. (P = 0.001).