Figure 2
From: Overexpression of LCMR1 is significantly associated with clinical stage in human NSCLC
Recombinant LCMR1 protein expression and polyclonal antibody preparation. M, protein marker; lane 1, pGEX-5T-LCMR1 before induction in E.coli; lane 2, pGEX-5T-LCMR1 after induction in E.coli; lane 3, precipitation after E.coli lysis; lane 4, clear supernatant after E.coli lysis; lane 5, GST-LCMR1 after purification; lane 6, GST-LCMR1 fusion protein recognized by GST antibody; lane 7, GST protein recognized by GST antibody; lane 8, GST-LCMR1 fusion protein recognized by LCMR1 polyclonal antibody. (lane 1-5, SDS-PAGE; lane 6-8, western blot)