Figure 4

Effect of miR-451 upregulation on the Akt signaling pathway. A. Western Blot analysis of pAkt (473), total Akt, Bcl-2 and Bax protein expression in mock A549, A549/miR-NC or A549/miR-451 cells. GAPDH was used as an internal control. B. Analysis of relative caspase-3 activity in mock A549, A549/miR-NC or A549/miR-451 cells. All experiments were performed in triplicate.