Figure 5

The effect of LS081 on colony formation of PC3 Cells. PC-3 cells in 10% FCS-RPMI1640 were seeded at a density of 500 cells/well into 6-well plates. After 24 hrs, cells were treated with 0.1% DMSO, 3 or 10 μM LS081 ± 10 μM ferric ammonium citrate for 48 hrs. The medium was replaced with 10% FCS-RPMI1640 and the cells were allowed to grow for ~ 10-14 days before Crystal violet staining and counting of colonies. Shown are the mean numbers of colonies ± SEM of 3-4 of independent observations with duplicates or triplicates for each observation. **: P < 0.01 compared to either Fe alone or 3 μM LS081 alone; ***: p < 0.001 compared to Fe alone or 10 μM LS081 alone by 1-way ANOVA with Newman-Keuls's posttests.