Nm23 knock-down promotes cell invasion and increases ITGA5 expression. Nm23 and ITGA5 were knocked down via siRNA to determine their effects on T47D cell invasion. (A) The invasion assay showed that alcohol and siNm23 independently increased cell invasion. ITGA5 knockdown by siRNA suppressed EtOH and siNm23-induced cell invasion in T47D cells. ITGA5 siRNA decreased cellular invasion. (B) Following siNm23 in T47D cells, mRNA expression of Nm23 was reduced 62% while ITGA5 mRNA expression increased relative to the siRNA control. siITGA5 in T47D cells resulted in a 65% knock-down of ITGA5 expression and Nm23 levels were not affected. Double siRNA of Nm23 and ITGA5 suppressed the expression of both to less than 40%. (C) Western blot shows expression of Nm23 and ITGA5 following siRNA. (*p < 0.05, as compared to the control cells).