Figure 4

Reduced UCH-L1 expression alters morphology of H838 cells and increases the number of apoptotic cells. A. Phase-contrast microscopy photographs of i) non-transfected H838 cells; ii) scrambled siRNA-treated H838 cells; iii) UCH-L1 siRNA-treated H838 cells. B. H & E staining of i) non-transfected H838 cells; ii) scrambled siRNA-treated H838 cells; iii) UCH-L1 siRNA-treated H838 cells. (Scale bar is equivalent to 15 μm). C. Number of apoptotic cells counted in 20 fields of H&E stained slides at 250× magnification.