Figure 4

Ad-bFGF-siRNA reduces the mitochondrial transmembrane potential (ΔΨm) and induces apoptosis in U251 cells. (A) Cytofluorimetric analysis using JC-1 staining demonstrated that Ad-bFGF-siRNA treatment (MOI = 100) induces depolarization of mitochondria. Percentages of cells with high ΔΨm (%) are shown in each column. Data are represented as mean ± SD of three replicates (**: P < 0.0005). Changes in ΔΨm were also detected by fluorescence microscopy. Magnification: 200×. Scale bar: 50 μm. Normal cells that have high ΔΨm show punctuate yellow fluorescence. Apoptotic cells show diffuse green fluorescence because of the decrease in mitochondrial membrane potential. (B) Western blot analysis revealed that Ad-bFGF-siRNA (MOI = 100 for 72 h) increases the expressions of Cytochrome C, Caspase3, and Bax.