Figure 2
Knock down of JMJD2A resulted in cell cycle change and proliferation inhibition. A. DNA contents of MDA-MB-231 cells treated in blank control group, negative control group and siRNA group by FCM. B. Column diagram analysis for the percentages of cells at each phase in three different groups: G0/G1 phase, S phase and G2/M phase. At G0/G1 phase, there was a significant difference in the percentage of cells in siRNA group compared with blank control group and negative control group respectively. At S phase, there was a significant difference in the percentage of cells in siRNA group compared with blank control group and negative control group respectively, while no significant differences in the percentages of cells at G2/M phase in the three groups. C. Column diagram analysis for the proliferation indexes (PI) calculated in three different groups. PI in siRNA group was significantly lower than that in blank control group and negative control group respectively. D. Column diagram analysis for the actual absorbance of three different groups, the mean actual absorbance of siRNA group was significantly lower than that of the blank control group and the negative control group, respectively. (*P < 0.05, compared with blank control group and negative control group respectively)