Figure 1

Effects of siRNA on DNMT1 mRNA and protein expression. (A): mRNA expression levels of DNMT1 in Hela and Siha cells were examined by qPCR. Compared with control group, Hela and Siha cells transfected with DNMT1-siRNA displayed lower level of mRNA expression (**P < 0.01). (B): DNMT1 protein levels in Hela and Siha cells were determined by western blot. The protein level of DNMT1 decreased significantly compared with control group and blank control. (1: transfection group (DNMT1-siRNA); 2: control group (negative siRNA); 3: blank group (Lipo2000), n = 3).