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Table 2 Primers for gene analysis

From: Combined analysis of mRNA expression of ERCC1, BAG-1, BRCA1, RRM1 and TUBB3 to predict prognosis in patients with non-small cell lung cancer who received adjuvant chemotherapy

Gene Accession Number Primer sequence(5'-3') Product length Tm
ERCC1 NM_001983.3 Forward 5'-CCCTGGGAATTTGGCGACGTAA-3' 273 bp 59°C
   Reverse 5'-CTCCAGGTACCGCCCAGCTTCC-3'   
BAG1 NM_004323.5 Forward 5'-GGCAGCAGTGAACCAGTTG-3' 242 bp 54.5°C
   Reverse 5'-GCTATCTTCTCCACAGACTTCTC-3'   
BRCA1 NM_007294.3 Forward 5'-AAGGTTGTTGATGTGGAGGAG-3' 208 bp 55.6°C
   Reverse 5'-CAGAGGTTGAAGATGGTATGTTG-3'   
RRM1 NM_001033.3 Forward 5'-TGGCCTTGTACCGATGCTG-3' 161 bp 57.5°C
   Reverse 5'-GCTGCTCTTCCTTTCCTGTGTT-3'   
TUBB3 NM_006086.3 Forward 5'-CGGATCAGCGTCTACTAC-3' 222 bp 49°C
   Reverse 5'-CACATCCAGGACCGAATC-3'   
β-actin NM_001101.3 Forward 5'-CTCGCGTACTCTCTCTTTCTGG-3' 334 bp 60°C
   Reverse 5'-GCTTACATGTCTCGATCCCACTTAA-3'