Table 2 Primers for gene analysis
Gene | Accession Number | Primer sequence(5'-3') | Product length | Tm |
|---|---|---|---|---|
ERCC1 | NM_001983.3 | Forward 5'-CCCTGGGAATTTGGCGACGTAA-3' | 273 bp | 59°C |
Reverse 5'-CTCCAGGTACCGCCCAGCTTCC-3' | ||||
BAG1 | NM_004323.5 | Forward 5'-GGCAGCAGTGAACCAGTTG-3' | 242 bp | 54.5°C |
Reverse 5'-GCTATCTTCTCCACAGACTTCTC-3' | ||||
BRCA1 | NM_007294.3 | Forward 5'-AAGGTTGTTGATGTGGAGGAG-3' | 208 bp | 55.6°C |
Reverse 5'-CAGAGGTTGAAGATGGTATGTTG-3' | ||||
RRM1 | NM_001033.3 | Forward 5'-TGGCCTTGTACCGATGCTG-3' | 161 bp | 57.5°C |
Reverse 5'-GCTGCTCTTCCTTTCCTGTGTT-3' | ||||
TUBB3 | NM_006086.3 | Forward 5'-CGGATCAGCGTCTACTAC-3' | 222 bp | 49°C |
Reverse 5'-CACATCCAGGACCGAATC-3' | ||||
β-actin | NM_001101.3 | Forward 5'-CTCGCGTACTCTCTCTTTCTGG-3' | 334 bp | 60°C |
Reverse 5'-GCTTACATGTCTCGATCCCACTTAA-3' |