Figure 4

RGC-32 mediates TGF-β-induced EMT in BxPC-3 cells. BxPC-3 cells were transfected with RGC-32 siRNA (siRGC-32) or negative control siRNA (siCtrl). 6 h later, cells were starved in serum-free RPMI-1640 for additional 6 h, followed by treatment with or without 10 ng/ml TGF-β1 for 72 h. The mRNA expression and protein expression of RGC-32, E-cadherin and vimentin were examined by qRT-PCR (A) and western blot (B and C) respectively. β-actin was used as an internal control. RGC-32 silencing significantly blocked TGF-β-induced EMT in BxPC-3 cells. *P < 0.05.