Figure 5

Effects of altered BMPR-IB expression on the mRNA and protein expression of p21, CDK2, CDK4, p27Kip1, Skp2 and p53 in human glioma cell lines. (A) Real-time RT-PCR was used to reveal alterations in the mRNA expression of p21, CDK2, CDK4, p27Kip1, Skp2 and p53 (values are expressed as the mean ± SD, n = 3. *, P < 0.05). (B) Western blot analysis showed alterations in the protein expression of p21, p27Kip1, Skp2 and p53 in these cell lines. Equal protein loading was monitored by hybridizing the same filter membrane with anti-beta-actin antibodies. (C) Statistical analysis of results from WB analysis. (Values are expressed as the mean ± SD, n = 3. *, P < 0.05).