Figure 3

The FACS analysis was performed after single-cell suspension obtained by enzymatic dissociation from xenografted tumors of NOG-EGFP mice. A) Two subpopulations indicating the cancer cells and eGFP-expressing cells were clearly distinguished. The collected cancer cells were dyed with phenotypic markers to evaluate the contamination rate of host cells in the collected cancer cells. Results of CD11c are shown as representative data of the phenotypic markers. B) The contamination rates of the phenotypic markers for murine stromal cells among the collected cancer cells are summarized as a table; note that only a few host cells are contaminated.