Figure 3
From: 5-Fluorouracil induces apoptosis in rat cardiocytes through intracellular oxidative stress
Modulation of intracellular levels of ROS. H9c2 and HT-29 were incubated with dihydroethidine and analyzed by flow cytometry as described in “Materials and Methods”. (A,C) Flow cytometric analysis of H9c2 (A) and HT-29 (C) cells treated with 5-FU alone or combined with LF or DOXO alone exposed to dihydroethidine used as a probe for measurement of O2−. (B,D) Representation of the ROS levels expressed as the percentage of mean fluorescence intensity (MFI) derived by dihydroethidine oxidation of H9c2 (B) and HT-29 (D) cells treated with 5-FU alone or combined with LF or DOXO alone. The experiments were repeated at least three times and gave always similar results. Bars, SDs.