Figure 4

Expression and biological activity of PHD3. (A) PHD3 mRNA was measured by quantitative real-time RT-PCR. Cells transfected with PHD3 significantly overexpressed PHD3, compared with the control groups (all P=0.00). (B and C) PHD3 protein was analyzed by western blot. Cells transfected with PHD3 significantly overexpressed PHD3, compared with the control groups (all P=0.00). Normal: no treatment, LP2000: Lipofectamine™ 2000, PC3.1: Lipofectamine™ 2000+pcDNA 3.1(+), PHD3: Lipofectamine™ 2000+pcDNA 3.1(+)-PHD3. ^#P<0.05 indicates statistically significant differences in comparison to PHD3-transfected cells.