Figure 1
From: Enforced effect of tk-MCP-1 fusion gene in ovarian cancer
The plasmid characterization and confirmation of expression of tk and MCP-1 by RT-PCR and western blot. A. The construction of the bicistronic recombinant replication-defective retroviruses vector pLXSN/tk-MCP-1, pLXSN/tk and pLXSN/MCP-1. B. Restriction enzyme analysis of pLXSN/tk-MCP-1 showed that tk and/or MCP-1 gene fragment had insert in the proper orientation in the vector of pLXSN, pLXSN/tk, pLXSN/MCP-1 and pLXSN/neo. a: EcoR I + BamH I, b: Nco I + BamH I, c: Sal I + BamH I, d: EcoR I, M: Marker. C. The expression of tk and MCP-1 protein were detected by western blot 48 h after transfection. a: SKOV3/tk. b: SKOV3/MCP-1. c: SKOV3/neo. d: SKOV3/tk-MCP-1.