A comparison of Direct sequencing, Pyrosequencing, High resolution melting analysis, TheraScreen DxS, and the K-ras StripAssay for detecting KRAS mutations in non small cell lung carcinomas

Table 3 False positive and false negative rates of the different methods

	Sequencing (n=131)	Pyrosequencing (n=131)	TheraScreen DxS (n=131)	K-ras StripAssay (n=131)	HRM (n=116)
False positives (1 - specificity)	0/110 (0 %)	0/110 (0 %)	0/110 (0 %)	6/110 (5 %)	2/96 (2 %)
False negatives (1 - sensitivity)	15/21 (71 %)	11/21 (52 %)	1/21 (5 %)	2/21 (10 %)	6/20 (30 %)

ISSN: 1756-9966