Figure 3

MiR-20a restoration in HCC cell lines induces apoptosis a SMMC-7721 and HepG2 cells transfected with miR-20a precursor were stained with FITC and PI. 20,000 cells were analyzed by flow cytometry. The LR quadrant represents the percentage of apoptotic cells (annexin V + and PI-) in the total cell population. Each type of cell was assayed in triplicate. All data were processed by Student’s t test and presented as mean ± SD. Asterisks indicate statistical significance of differences in the apoptosis rate of cells between miR-20a precursor transfected and control oligonucleotide transfected cells (P < 0.05; Student’s t test).