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Table 2 Effects of aqeous gall extract and luteolin on global methylated DNA in HeLa cells

From: Limoniastrum guyonianum aqueous gall extract induces apoptosis in human cervical cancer cells involving p16INK4A re-expression related to UHRF1 and DNMT1 down-regulation

Average of absorbance (nm)

Methylated DNA (% of control)

MC

0.662 ± 0.030

259.90* ± 4.9

C

0.283 ± 0.001

100.00

G200

0.152 ± 0.003

53.53* ± 1.52

L25

0.163 ± 0.005

57.60 * ± 2.29

  1. Total DNA was isolated from HeLa cancer cells using QIAamp® DNA Kit. the content of methylated DNA was determined using 200 ng of DNA from untreated cells (C), treated cells with 200 μg/ml of G extract (G200 or with 25 μM of luteolin(L25) for 48 hours and the commercial methylated control (MC) (Imprint Methylated DNA Quantification Kit) Values are means ± S.E.M. of three independent experiments. Statistically significant, *P < 0.001 (versus the untreated cells).
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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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