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Table 1 Questionnaire results from the 16 participant centers

From: External Quality Assessment (EQA) program for the preanalytical and analytical immunohistochemical determination of HER2 in breast cancer: an experience on a regional scale

  N (%)
N° annual HER2 determination  
<100 4 (25%)
100-500 10 (62.5%)
>500 2 (12.5%)
Material  
Paraffin embedded tissue 16 (100%)
Type of fixation  
Buffered formalin 12 (75%)
Formalin 2 (12.5%)
Other 2 (12.5%)
Time of tissue fixation  
12 hours 2 (12.5%)
24 hours 10 (62.5%)
Other 4 (25%)
Time from cutting to IHC  
24 hours 10 (62.5%)
1 week 4 (25%)
other 2 (12.5%)
Slide storage  
37°C 5 (31%)
Room temperature 11 (69%)
Immunostaining procedure  
Automated 11 (69%)
Manual 5 (31%)
Type of reagent  
A0485 PoAb 11 (69%)
CB11 MoAb 4 (25%)
Herceptest 1 (6%)
Chromogen  
DAB 16 (100%)
Evaluation  
Optical microscope 15 (94%)
Optical microscope + Image analyzer 1 (6%)
Evaluation criteria  
Score and % of positive cells 10 (62.5%)
Score 6 (27.5%)