Figure 3

SPAG9 gene silencing significantly inhibited migration and invasion ability of MDA-MB-231 cells. (a) Representative photomicrograph showed reduced number of migrated cells transfected with SPAG9 siRNA as compared to control siRNA transfected cells. A histogram shows significant reduction (P < 0.005) in the number of migrated cells transfected with SPAG9 siRNA as compared to control siRNA transfected cells. Observations based on three experimental triplicates. (b) Knockdown of SPAG9 gene significantly reduced invasion of MDA-MB-231 cells through the Matrigel. Representative photomicrograph showed SPAG9 siRNA transfected cells exhibit reduced invasion abilities through Matrigel-coated Transwell filters as compared to control siRNA transfected cells. (c) A histogram shows significant reduction (P < 0.005) in the number of invaded cells in SPAG9 siRNA transfected MDA-MB-231 cells as compared to control siRNA transfected cells. Columns indicate mean (n = 3); bars, standard error. *; P < 0.005 statistically significant compared with control siRNA. These results are representative of three independent experiments performed in triplicates.