Figure 4From: SMO expression level correlates with overall survival in patients with malignant pleural mesotheliomaQuantitative RT-PCR analysis of Shh pathway effectors in mesothelioma cell lines treated with cyclopamine. Cells were treated with 15 uM cyclopamine for 72Â hrs. RNA was then collected for cDNA synthesis and quantitative PCR. Actin was used as an internal control for normalization.Back to article page