EZH2 knockdown inhibits the growth of SUM149 and FC-IBC-02S cells in vivo in SCID mice. A and B. Control or shEZH2 expressing FC-IBC-02S (1 × 106) and SUM149 (3 × 106) cells were orthotopically transplanted into the mammary fat pads of SCID mice. Nine weeks (FC-IBC-02S cells) and six weeks (SUM149 cells) post injection, tumors were removed from mice and imaged. C (FC-IBC-02S) and D ( SUN149) cells, tumor size was measured by caliper measurements. Data shown represent mean tumor volume with SEM, p value compared with control. E (FC-IBC-02S) and F (SUN149), western blot assay of EZH2 expression from pooled FC-IBC-02S and SUM149 xenografted tumors.