Downregulation of hMOF is accompanied by increased CA9 in ccRCC. A-B. Relative mRNA expression levels of hMOF and CA9 in ccRCC. Total RNA was isolated from sixteen paired clinical ccRCC and adjacent kidney tissues. Relative mRNA expression levels of hMOF and CA9 were analized by quantitative RT-PCR. Error bars represent the standard error of the mean of 3 independent experiments. Student’s t-test was performed to compare the difference between ccRCC and normal tissues. C. Expression patterns of hMOF and CA9 mRNAs in ccRCC and its corresponding adjacent kidney tissues. Expression is displayed as a ratio of expression of hMOF or CA9 gene in ccRCC versus matched normal tissues. Each bar is the log2 value of the ratio of hMOF or CA9 expression levels between ccRCC and matched normal tissues from the same patients. Bar value >1 represents >2-fold increased, whereas bar value <−1, represents >2-fold decreased. D. Protein expression levels of hMOF and its H4K16Ac status in ccRCC. Aliquots of whole cell extracts from sixteen selected ccRCC tumor samples and its corresponding adjacent tissues were analyzed by western blotting. The blots were then scanned and quantified with Quantity One software. The significant difference is expressed as *p<0.05, **p<0.01.