Figure 4
From: Everolimus-induced human keratinocytes toxicity is mediated by STAT3 inhibition
Effects of various STAT3 inhibitors on everolimus-mediated signal transduction in HaCaT cells. (A) Alteration in signal transduction of STAT3. HaCaT cells were incubated in medium containing everolimus at the indicated concentrations for 2Â h (1): after pretreatment with 10Â ÎĽM stattic for 20Â min or (2): coincubation with everolimus and 10Â ÎĽM STA-21 or (3) vehicle alone (DMSO). (B) Alteration in signal transduction of STAT3. HaCaT cells were incubated in medium containing 10Â ÎĽM everolimus at the indicated time. Total cell lysates were separated by SDS-PAGE and electrotransferred to PVDF membranes. Various proteins and phosphorylation levels were evaluated by immunoblotting assay with specific antibodies.