GA impairs the migratory activity of stimulated MO-DCs. Groups of MO-DCs were generated as described (see legend of Figure 2). (a) Expression of the actin-bundling protein Fscn1 was assessed by intracellular flow cytometry. Data represent the means ± SEM of MFI intensities of 6 independent experiments. (b) Spontaneous migration of MO-DC populations in 3D collagen matrices was monitored for 6 h by time lapse analysis in intervals of 2 min. Graphs represent the means ± SEM of around 80 MO-DCs per group individually tracked in two independent experiments compiled. Statistical significance: *versus unstimulated MO-DCs, #GA-treated MO-DCs at stimulated versus unstimulated state, and $GA-treated versus untreated MO-DCs at stimulated state (*,$P < 0.05, **P < 0.01, ***,###,$$$P < 0.001).