Figure 4

Expression of p53, c-myc, p21 and p27 in HCT-116 and MSTO cells. Cells were cultured in the absence or presence of CF (1:200) for the indicated time and whole cell lysates were analyzed by western blot. Data representing three independent experiments with similar results, indicate an upregulation of p53, p21 and p27 and a downregulation of c-myc in HCT-116 and MSTO cell upon CF treatment vs untreated cells. γ tubulin was examined as a loading control.