Skip to main content

Advertisement

Archived Comments for: Aquaporin 3 promotes epithelial-mesenchymal transition in gastric cancer

Back to article

  1. AQP3, one of the aquaglyceroporins, might cause EMT via metabolic reprogramming.

    Go Yoshida, Tokyo Medical and Dental University

    29 July 2014

    Accumulating evidence suggests that aquaporins (AQPs) play a crucial role not only in the homeostasis of the normal tissue but also in the development and progression of autoimmune disorders and malignant neoplasms. AQP-3, -7, -9 belong to the aquaglyceropolins, which permit the penetration of glycerol as well as water through the lipid cellular membrane. It has been reported that TNF-alpha decreases the expression of AQP-3 through the activation of p38 and extracellular signal-regulated kinase (ERK) signaling pathways (Mol Cell Biol 2008;28:326-332.). However, less is known about which subtype of aquaglyceropolin is highly expressed in cancer cells.

    In this article, the authors demonstrated, using two kinds of gastric cancer cell lines, that si-RNA-mediated AQP3 depletion induced mesenchymal-epithelial transition (MET) or prevented EMT. Activation of EGFR signal transduction resulted in the up-regulation of AQP-3. Activation of EGFR signal pathway partially rescued AQP3 depletion in terms of EMT in Figure3-4. In Figure5, the authors failed to demonstrate whether or not overexpression of AQP3 was really responsible for EMT in gastric cancer cells.

    Further, the authors should have evaluated phosphorylated-Akt as well as Akt and other EMT-inducer transcription factors other than Snail in Figure6. As far as I have performed several experiments on AQP3 in cancer tissues, EGFR signal pathway up-regulated AQP3. AQP3, 7, and 9 contribute to the uptake of glycerol, thereby promoting the lipid metabolism.

    That is why the following two points exist the authors should more investigate; Firstly, the crosstalk between EGFR signal and PI3K/Akt/Snail axis should be focused on. And secondly, they should study whether or not AQP3-induced metabolic reprogramming is related to EMT.

    Competing interests

    No Competing Interests Exist.

Advertisement