Figure 4
From: In silico and Ex vivo approaches identify a role for toll-like receptor 4 in colorectal cancer
Immunofluorescent staining of TMAs. A) Low power (10x) view of NCI TMA slide stained for TLR4 (green), intestinal epithelium/pan-cytokeratin (red), and nucleus/DAPI (blue). B) Representative tissue cores from normal (I), adenomatous polyps (II), and CRC (III and IV) are shown. C and D) TLR4 staining score by tissue type and tissue compartment (stroma vs epithelium) are shown. C) TLR4 staining in the tumor stroma had a significantly higher average intensity score for stages 3 and 4 CRC when compared to stage 1. D) TLR4 staining in the tumor epithelium had a significantly higher average intensity score for stages 2 and 3 when compared to stage 1. E) TLR4 staining by compartment broken down by stage (controlling for grade) and grade (controlling for stage).