Table 1 The oligonucleotides used in this study
From: MicroRNA-320c inhibits tumorous behaviors of bladder cancer by targeting Cyclin-dependent kinase 6
Namea | Sequence (5′- > 3′) |
|---|---|
miR-320c mimics (sense) | AAAAGCUGGGUUGAGAGGGU |
NC (sense) | ACUACUGAGUGACAGUAGA |
miR-320c inhibitor | ACCCUCUCAACCCAGCUUUU |
microRNA inhibitor NC | CAGUACUUUUGUGUAGUACAA |
siCDK6 (sense) | CUGGAAAGGUGCAAAGAAAdTdT |
miR-320c F | AAAAGCTGGGTTGAGAGGGT |
U6 F | TGCGGGTGCTCGCTTCGGCAGC |
CDK6 F | GGATAAAGTTCCAGAGCCTGGAG |
CDK6 R | GCGATGCACTACTCGGTGTGAA |
GAPDH F | AAGGTGAAGGTCGGAGTCA |
GAPDH R | GGAAGATGGTGATGGGATTT |
CDK6-Wt F | cAATCAATGCAAGAGTGATTGCAGCTTTATGTTCATTTGTTTGTTTGTTg |
CDK6-Wt R | tcgacAACAAACAAACAAATGAACATAAAGCTGCAATCACTCTTGCATTGATTgagct |
CDK6-Mut F | cAATCAATGCAAGAGTGATTGgtcgaaatTGTTCATTTGTTTGTTTGTTg |
CDK6-Mut R | tcgacAACAAACAAACAAATGAACAatttcgacCAATCACTCTTGCATTGATTgagct |