Figure 5

Orai1 regulated focal adhesion turnover and EMT-like by modulating Pyk2 phosphorylation. (A) Representative Orai1 (green) and vinculin (red) staining in U251 and SNB19 cells transfected with shControl, shOrai1, Orai1 rescue, or shPyk2. Magnification, ×1000. (B) Proportions of cells with large focal adhesions are presented as means ± SDs of nine different microscopic visions from three independent experiments. **P < 0.01. (C) Western blot analysis of E-cadherin, N-cadherin, and vimentin expression in U251 and SNB19 cells transfected with shControl, shOrai1, Orai1 rescue, or shPyk2.