Table 1 Intracellular amino acid concentrations (nmol/100 μl) of L3.3 and MIA-PaCa-2 after a 24 hr incubation in HBSS, and 4 hours later in the absence or presence of exogenous citrulline and aspartate (1 mM)
L3.3 | Â | Â | Â | Â |
|---|---|---|---|---|
|  | Baseline | T = 4 hrs (HBSS) | T = 4 hrs (+cit/asp) | Effect of cit/asp addition |
Citrulline | N.D. | N.D. | 2.375 | 2.375 |
Arginine | 0.778 | 0.624 | 6.511 | 5.887 |
Aspartate | 7.565 | 6.310 | 5.141 | −1.169 |
Ammonia | 1.680 | 1.105 | 1.228 | .123 |
Lysine | 0.567 | 0.450 | 0.432 | -.018 |
Phosphoserine | 0.295 | 0.300 | 0.296 | −0.004 |
AE-cys (int stand) | 3.050 | 3.218 | 3.206 | Â |
MIA-PaCa-2 | Â | Â | Â | Â |
|  | Baseline | T = 4 hrs (HBSS) | T = 4 hrs (+cit/asp) | Change |
Citrulline | N.D. | N.D. | 2.513 | 2.513 |
Arginine | 0.768 | 0.949 | 0.802 | −0.147 |
Aspartate | 2.005 | 2.040 | 1.942 | −0.098 |
Ammonia | 4.316 | 4.162 | 4.338 | .176 |
Lysine | 0.537 | 0.618 | 0.572 | -.046 |
Phosphoserine | 0.261 | 0.219 | 0.189 | −0.030 |
AE-cys (int stand) | 3.188 | 3.311 | 3.206 | Â |