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Table 1 Intracellular amino acid concentrations (nmol/100 μl) of L3.3 and MIA-PaCa-2 after a 24 hr incubation in HBSS, and 4 hours later in the absence or presence of exogenous citrulline and aspartate (1 mM)

From: Pegylated arginine deiminase synergistically increases the cytotoxicity of gemcitabine in human pancreatic cancer

L3.3     
  Baseline T = 4 hrs (HBSS) T = 4 hrs (+cit/asp) Effect of cit/asp addition
Citrulline N.D. N.D. 2.375 2.375
Arginine 0.778 0.624 6.511 5.887
Aspartate 7.565 6.310 5.141 −1.169
Ammonia 1.680 1.105 1.228 .123
Lysine 0.567 0.450 0.432 -.018
Phosphoserine 0.295 0.300 0.296 −0.004
AE-cys (int stand) 3.050 3.218 3.206  
MIA-PaCa-2     
  Baseline T = 4 hrs (HBSS) T = 4 hrs (+cit/asp) Change
Citrulline N.D. N.D. 2.513 2.513
Arginine 0.768 0.949 0.802 −0.147
Aspartate 2.005 2.040 1.942 −0.098
Ammonia 4.316 4.162 4.338 .176
Lysine 0.537 0.618 0.572 -.046
Phosphoserine 0.261 0.219 0.189 −0.030
AE-cys (int stand) 3.188 3.311 3.206  
  1. Lysine and phosphoserine are shown to demonstrate no other significant differences in amino acid levels, and AE-cys is an internal control spiked into every sample at 3 nmoles. N.D. = not detectable.